In order to identify the polymorphism the parotid isoelectric focusing variant protein(PIF),as an integrating part of systemic study about parotid salivary proteins, this study was conducted. One-hundred and fifty healthy adult Koreans between
the
ages
of 20 and29were chosen, and their parotid saliva was collected. The parotid saliva went under isolelectric focusing on a urea-polyacrylamide slap gel(pH 3.5-5.2) for 5 hours. Then, it was stained with Coomasie Brilliant Blue R-250/20%
Trichloroacetic
acid (TCA)and again was destained with 20% TCA soultion.
The phenotype frequency of each isoelectric focusing variant protein(PIF) were obtained, and the subjects were divied into the indigenous family name group and immigrated family name group. The phenotype and gene frequency of each group were
compared,
and the following results were obtained:
1. The phenotypes of parotid isoelectric focusing variant protein(PIF) observed in parotid saliva of the 150 Koreans were PIF(+) in 140 people(93.3%) and PIF(-) in 10 people (6.7%).
2. The gene frequency of PIF+ was 0.742, and that of PIF- was 0.258.
3. Of the 150 Koreans, the frequencies of the indigenous family name group were 0.733 in PIF+ and 0.267 in PIF-; those of the immigrated family name group were 0.796 and 0.234.
4. The gene frequency of the entire Korean group was closer to that of indigenous family name group than the immigrated family name group.
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